Slit lamp
Biomicroscopy:-
Presented By:-Anmol Singh
Boptom 2k24
UPUMS SAIFAI ETAWAH
Part :- 02
Types of Slit Lamp :-
1. Zeiss Type (Parallel Viewing)
● Both illumination and observation systems
move independently.
2. Haag-Streit Type (Fixed Illumination, Movable
Microscope)
● Most commonly used design in clinics.
Allows variable angle of illumination from 0°–90°.
1. Zeiss Type (Parallel Viewing)
2. Haag-Streit Type
Magnification and Filters:-
(A) Magnification
● Varies by changing eyepieces or drum
selector:
● Low (6×–10×): For general scanning.
● Medium (16×): For cornea and lens.
● High (25×–40×): For detailed corneal layers
and cells.
(B) Filters
1. Cobalt Blue Filter: Used for fluorescein staining (e.g.,
corneal abrasions, tear film break-up time).
2. Red-Free (Green) Filter: Enhances visibility of blood
vessels and nerve fibers.
3. Neutral Density Filter: Reduces brightness for patient
comfort.
4. Diffuser Filter: For general illumination and
eyelid/conjunctiva view.
5. Yellow Barrier Filter: Increases contrast during
fluorescein staining.
Preparation for Examination:-
1. Seat the patient comfortably.
2. Adjust chin rest and forehead band.
3. Ensure room illumination is dim for better
contrast.
4. Examiner’s eye level should match the patient’s.
5. Clean lenses and ensure filters and slits are
functioning.
6. Explain the procedure to the patient.
Technique of Examination:
(Step-by-Step)
1. Begin with low magnification and wide beam
for general survey.
2. Then, reduce slit width and increase
magnification for detailed observation.
3. Examine systematically:
● Eyelids and lashes
● Conjunctiva and sclera
● Cornea (epithelium, stroma, endothelium)
● Anterior chamber
● Iris and pupil
● Lens
● (Optional) Anterior vitreous
4. Move from superficial to deeper structures.
Methods of Illumination:-
Illumination is the heart of slit lamp examination. Each
method reveals specific details:
(A) Diffuse Illumination:-
● Broad beam with diffuser filter.
● Used for overview of external structures like
lids, conjunctiva, and cornea.
(B) Direct Focal Illumination:-
● Beam and microscope focused on same point.
● Used to study corneal layers (epithelium,
stroma, endothelium).
● Types:
● Optical Section: Very narrow slit → for
assessing depth and transparency.
● Parallelepiped Beam: Slightly wider beam →
highlights surface and texture.
(C) Specular Reflection
● The light beam is directed at equal
angle to that of observation.
●
● Produces mirror-like reflection from
smooth surfaces such as corneal
endothelium and tear film.
(D) Retro-Illumination:-
● Light reflected from the iris or
fundus illuminates the structure
from behind.
● Useful to detect opacities, keratic
precipitates, or lens changes.
(E) Indirect Illumination:-
● Observation is made adjacent to the
illuminated area.
● Helps in detecting microcysts,
infiltrates, or foreign bodies.
(F) Sclerotic Scatter:-
● The slit beam is directed at temporal
limbus, and light scatters within the
cornea.
● Any opacity or edema becomes
visible due to scattered light.
Structures Examined:-
1. Eyelids & Lashes – Position, inflammation, meibomian gland
openings.
2. Conjunctiva & Sclera – Redness, discharge, follicles, papillae.
3. Cornea – Clarity, epithelial defects, edema, infiltrates.
4. Anterior Chamber – Depth, cells, flare, hypopyon, hyphema.
5. Iris – Texture, color, neovascularization, synechiae.
6. Pupil – Shape, reaction to light, size abnormalities.
7. Lens – Transparency, opacities, position (for cataract detection).
8. Anterior Vitreous – Floaters, hemorrhage, cells.
Clinical Applications:-
● Diagnosis of anterior segment diseases: conjunctivitis,
keratitis, uveitis.
● Contact lens fitting & evaluation.
● Measurement of anterior chamber depth before tonometry
or surgery.
● Detection of corneal foreign bodies or abrasions.
● Assessment of cataract and lens opacities.
● Pre- and post-operative examination (especially in
cataract and LASIK).
● Observation of ocular surface and tear film stability.
Special Attachments:-
● Applanation Tonometer (Goldmann): For
intraocular pressure measurement.
● Fundus Lens (Volk/90D/78D): To visualize
posterior segment.
● Camera Attachment: For anterior segment
photography.
● Fluorescein and Specular Microscopy
Modules.
Advantages:-
● Provides 3D stereoscopic view.
● Enables layer-by-layer analysis of transparent
media.
● High magnification and contrast for
micro-level evaluation.
● Can be combined with diagnostic attachments
(tonometry, gonioscopy, etc.).
Limitations:-
● Limited visualization of posterior
segment.
● Requires patient cooperation.
● Bright light may cause discomfort.
● Expensive and not portable
compared to handheld devices.
Precautions:-
● Clean lenses and chin rest regularly.
● Avoid high illumination for prolonged duration.
● Proper disinfection to prevent cross-infection.
● Recheck alignment and calibration
periodically.
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  • 1.
    Slit lamp Biomicroscopy:- Presented By:-AnmolSingh Boptom 2k24 UPUMS SAIFAI ETAWAH Part :- 02
  • 2.
    Types of SlitLamp :- 1. Zeiss Type (Parallel Viewing) ● Both illumination and observation systems move independently. 2. Haag-Streit Type (Fixed Illumination, Movable Microscope) ● Most commonly used design in clinics. Allows variable angle of illumination from 0°–90°.
  • 3.
    1. Zeiss Type(Parallel Viewing)
  • 4.
  • 5.
    Magnification and Filters:- (A)Magnification ● Varies by changing eyepieces or drum selector: ● Low (6×–10×): For general scanning. ● Medium (16×): For cornea and lens. ● High (25×–40×): For detailed corneal layers and cells.
  • 6.
    (B) Filters 1. CobaltBlue Filter: Used for fluorescein staining (e.g., corneal abrasions, tear film break-up time). 2. Red-Free (Green) Filter: Enhances visibility of blood vessels and nerve fibers. 3. Neutral Density Filter: Reduces brightness for patient comfort. 4. Diffuser Filter: For general illumination and eyelid/conjunctiva view. 5. Yellow Barrier Filter: Increases contrast during fluorescein staining.
  • 7.
    Preparation for Examination:- 1.Seat the patient comfortably. 2. Adjust chin rest and forehead band. 3. Ensure room illumination is dim for better contrast. 4. Examiner’s eye level should match the patient’s. 5. Clean lenses and ensure filters and slits are functioning. 6. Explain the procedure to the patient.
  • 8.
    Technique of Examination: (Step-by-Step) 1.Begin with low magnification and wide beam for general survey. 2. Then, reduce slit width and increase magnification for detailed observation.
  • 9.
    3. Examine systematically: ●Eyelids and lashes ● Conjunctiva and sclera ● Cornea (epithelium, stroma, endothelium) ● Anterior chamber ● Iris and pupil ● Lens ● (Optional) Anterior vitreous 4. Move from superficial to deeper structures.
  • 10.
    Methods of Illumination:- Illuminationis the heart of slit lamp examination. Each method reveals specific details: (A) Diffuse Illumination:- ● Broad beam with diffuser filter. ● Used for overview of external structures like lids, conjunctiva, and cornea.
  • 12.
    (B) Direct FocalIllumination:- ● Beam and microscope focused on same point. ● Used to study corneal layers (epithelium, stroma, endothelium). ● Types: ● Optical Section: Very narrow slit → for assessing depth and transparency. ● Parallelepiped Beam: Slightly wider beam → highlights surface and texture.
  • 15.
    (C) Specular Reflection ●The light beam is directed at equal angle to that of observation. ● ● Produces mirror-like reflection from smooth surfaces such as corneal endothelium and tear film.
  • 17.
    (D) Retro-Illumination:- ● Lightreflected from the iris or fundus illuminates the structure from behind. ● Useful to detect opacities, keratic precipitates, or lens changes.
  • 22.
    (E) Indirect Illumination:- ●Observation is made adjacent to the illuminated area. ● Helps in detecting microcysts, infiltrates, or foreign bodies.
  • 23.
    (F) Sclerotic Scatter:- ●The slit beam is directed at temporal limbus, and light scatters within the cornea. ● Any opacity or edema becomes visible due to scattered light.
  • 26.
    Structures Examined:- 1. Eyelids& Lashes – Position, inflammation, meibomian gland openings. 2. Conjunctiva & Sclera – Redness, discharge, follicles, papillae. 3. Cornea – Clarity, epithelial defects, edema, infiltrates. 4. Anterior Chamber – Depth, cells, flare, hypopyon, hyphema. 5. Iris – Texture, color, neovascularization, synechiae. 6. Pupil – Shape, reaction to light, size abnormalities. 7. Lens – Transparency, opacities, position (for cataract detection). 8. Anterior Vitreous – Floaters, hemorrhage, cells.
  • 27.
    Clinical Applications:- ● Diagnosisof anterior segment diseases: conjunctivitis, keratitis, uveitis. ● Contact lens fitting & evaluation. ● Measurement of anterior chamber depth before tonometry or surgery. ● Detection of corneal foreign bodies or abrasions. ● Assessment of cataract and lens opacities. ● Pre- and post-operative examination (especially in cataract and LASIK). ● Observation of ocular surface and tear film stability.
  • 28.
    Special Attachments:- ● ApplanationTonometer (Goldmann): For intraocular pressure measurement. ● Fundus Lens (Volk/90D/78D): To visualize posterior segment. ● Camera Attachment: For anterior segment photography. ● Fluorescein and Specular Microscopy Modules.
  • 29.
    Advantages:- ● Provides 3Dstereoscopic view. ● Enables layer-by-layer analysis of transparent media. ● High magnification and contrast for micro-level evaluation. ● Can be combined with diagnostic attachments (tonometry, gonioscopy, etc.).
  • 30.
    Limitations:- ● Limited visualizationof posterior segment. ● Requires patient cooperation. ● Bright light may cause discomfort. ● Expensive and not portable compared to handheld devices.
  • 31.
    Precautions:- ● Clean lensesand chin rest regularly. ● Avoid high illumination for prolonged duration. ● Proper disinfection to prevent cross-infection. ● Recheck alignment and calibration periodically.
  • 33.
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